Running into a strange one, we are suddenly seeing reports that Office apps won;t load but it seems to affect on a subset of server VDAs all from the same image. This just started, a reboot seems to help for a while then it starts to fail. We see the following error: "Error: The application can't start because AppVIsvSubsystems32.dll is missing from your computer". This is affecting two different images with different Office layers, one was recently updated and the other wasn't. It's quite possible this has nothing to do with App layering but we are stuck and looking for answers and figured I'd start here. I found this link, but the last post has a dead link which appears to be what helped. https://community.citrix.com/forums/topic/235330-error-the-application-cant-start-because-appvisvsubsystems32dll-is-missing-from-your-computer/