Daniel Lundin
06/26/2026, 3:07 PMPhil Ewels
06/26/2026, 4:04 PMDaniel Lundin
06/26/2026, 5:21 PMPhil Ewels
06/26/2026, 7:09 PMDaniel Lundin
06/26/2026, 7:12 PMPhil Ewels
06/26/2026, 10:12 PMPhil Ewels
06/26/2026, 10:12 PMNFCORE_METATDENOVO:METATDENOVO:MEGAHIT_INTERLEAVEDPhil Ewels
06/26/2026, 10:13 PMError executing process > 'NFCORE_METATDENOVO:METATDENOVO:MEGAHIT_INTERLEAVED (megahit_assembly)'
Caused by:
Missing output file(s) `megahit_out/megahit_assembly.contigs.fa.gz` expected by process `NFCORE_METATDENOVO:METATDENOVO:MEGAHIT_INTERLEAVED (megahit_assembly)`
Command executed:
megahit \
--12 ERR978615.processed.fastq.gz,ERR978616.processed.fastq.gz,ERR978614.processed.fastq.gz,ERR978612.processed.fastq.gz \
\
-t 1 \
-m 214748364800 \
\
--out-prefix megahit_assembly
pigz \
--no-name \
-p 1 \
\
megahit_out/*.fa \
megahit_out/intermediate_contigs/*.fa
Command exit status:
0
Command output:
(empty)
Command error:
2026-06-26 14:45:58 - MEGAHIT v1.2.9
2026-06-26 14:45:59 - Using megahit_core with POPCNT and BMI2 support
2026-06-26 14:45:59 - Convert reads to binary library
2026-06-26 14:47:10 - b'INFO sequence/io/sequence_lib.cpp : 75 - Lib 0 (/fusion/s3/nf-core-awsmegatests/work/metatdenovo/work-4d0307aa7d34575aa7891a7b4996ffe19e774cd9/9f/467bd1fc700aad17bed2e84015d437/ERR978615.processed.fastq.gz): interleaved, 40525544 reads, 101 max length'
2026-06-26 14:48:31 - b'INFO sequence/io/sequence_lib.cpp : 75 - Lib 1 (/fusion/s3/nf-core-awsmegatests/work/metatdenovo/work-4d0307aa7d34575aa7891a7b4996ffe19e774cd9/9f/467bd1fc700aad17bed2e84015d437/ERR978616.processed.fastq.gz): interleaved, 45305414 reads, 101 max length'
2026-06-26 14:50:59 - b'INFO sequence/io/sequence_lib.cpp : 75 - Lib 2 (/fusion/s3/nf-core-awsmegatests/work/metatdenovo/work-4d0307aa7d34575aa7891a7b4996ffe19e774cd9/9f/467bd1fc700aad17bed2e84015d437/ERR978614.processed.fastq.gz): interleaved, 83969516 reads, 101 max length'
2026-06-26 14:53:40 - b'INFO sequence/io/sequence_lib.cpp : 75 - Lib 3 (/fusion/s3/nf-core-awsmegatests/work/metatdenovo/work-4d0307aa7d34575aa7891a7b4996ffe19e774cd9/9f/467bd1fc700aad17bed2e84015d437/ERR978612.processed.fastq.gz): interleaved, 91205786 reads, 101 max length'
2026-06-26 14:53:40 - b'INFO utils/utils.h : 152 - Real: 461.8109\tuser: 129.5027\tsys: 36.2367\tmaxrss: 281384'
2026-06-26 14:53:40 - k-max reset to: 119
2026-06-26 14:53:40 - Start assembly. Number of CPU threads 1
2026-06-26 14:53:40 - k list: 21,29,39,59,79,99,119
2026-06-26 14:53:40 - Memory used: 214748364800
2026-06-26 14:53:40 - Extract solid (k+1)-mers for k = 21
2026-06-26 16:56:58 - Build graph for k = 21
2026-06-26 17:03:01 - Assemble contigs from SdBG for k = 21
2026-06-26 17:52:17 - Local assembly for k = 21
2026-06-26 17:59:32 - Extract iterative edges from k = 21 to 29
2026-06-26 18:20:10 - Build graph for k = 29
2026-06-26 18:23:17 - Assemble contigs from SdBG for k = 29
2026-06-26 18:42:27 - Local assembly for k = 29
Work dir:
<s3://nf-core-awsmegatests/work/metatdenovo/work-4d0307aa7d34575aa7891a7b4996ffe19e774cd9/9f/467bd1fc700aad17bed2e84015d437>
Container:
<http://wave.seqera.io/wt/f2e9c27936b7/biocontainers/mulled-v2-0f92c152b180c7cd39d9b0e6822f8c89ccb59c99:8ec213d21e5d03f9db54898a2baeaf8ec729b447-0|wave.seqera.io/wt/f2e9c27936b7/biocontainers/mulled-v2-0f92c152b180c7cd39d9b0e6822f8c89ccb59c99:8ec213d21e5d03f9db54898a2baeaf8ec729b447-0>
Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`Phil Ewels
06/26/2026, 11:17 PMPhil Ewels
06/26/2026, 11:19 PMPhil Ewels
06/26/2026, 11:19 PMPhil Ewels
06/26/2026, 11:22 PM