Good morning and thank you very much for the RNAsp...
# rnasplice
c
Good morning and thank you very much for the RNAsplice NF-Core workflow. I'm having a problem that I'm not sure how to correct and appreciate any help you can give. In an attempt to help myself, I downloaded the SUPPA software from GitHub and successfully ran suppa.py and generated the *.clustvec files, but can't get to work as part of the NF workflow. Here are the relevant parts (I think) of the log:
Dec-15 121244.699 [TaskFinalizer-5] ERROR nextflow.processor.TaskProcessor - Error executing process > 'NFCORE_RNASPLICERNASPLICESUPPA_SALMON:CLUSTEREVENTS_IOE (RoKD-CONTROL)'
Caused by:
Missing output file(s)
*.clustvec
expected by process
NFCORE_RNASPLICE:RNASPLICE:SUPPA_SALMON:CLUSTEREVENTS_IOE (RoKD-CONTROL)
Command executed:
suppa.py \
clusterEvents \
--dpsi KO-CONTROL_local_diffsplice.dpsi \
--psivec KO-CONTROL_local_diffsplice.psivec \
--dpsi-threshold 0.05 \
--eps 0.05 \
--metric euclidean \
--min-pts 20 \
--groups 1-2,3-4 \
--clustering DBSCAN \
-o RoKD-CONTROL_local_cluster
cat <<-END_VERSIONS > versions.yml
"NFCORE_RNASPLICERNASPLICESUPPA_SALMON:CLUSTEREVENTS_IOE":
suppa: $(python -c "import pkg_resources; print(pkg_resources.get_distribution('suppa').version)")
END_VERSIONS
Command exit status:
0
Command output:
Number of clustered events: 419/691 (60.636758%)
Cluster 0 contains 89 events
Cluster 1 contains 80 events
Cluster 2 contains 39 events
Cluster 5 contains 85 events
Cluster 3 contains 95 events
Cluster 4 contains 31 events
The RMSSTD score for cluster 0 is 0.193844
The RMSSTD score for cluster 1 is 0.152982
The RMSSTD score for cluster 2 is 0.107187
The RMSSTD score for cluster 5 is 0.178010
The RMSSTD score for cluster 3 is 0.163208
The RMSSTD score for cluster 4 is 0.189824
The average silhouette score is : 0.969885
Command error:
WARNING: Not virtualizing pid namespace by configuration
Again thank you for your help.
l
I'm seeing the same error
Copy code
Error executing process > 'NFCORE_RNASPLICE:RNASPLICE:SUPPA_SALMON:CLUSTEREVENTS_IOI (MUT-CTRL)'

Caused by:
  Missing output file(s) `*.clustvec` expected by process `NFCORE_RNASPLICE:RNASPLICE:SUPPA_SALMON:CLUSTEREVENTS_IOI (MUT-CTRL)`
I'm running Nextflow 25.10.2 and rnasplice 1.0.4