Hello dear nf-core community ! I would like some h...
# rnasplice
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Hello dear nf-core community ! I would like some help and suggestions on why the TEST of nf-core/rnasplice would give this error on clustering from SUPPA-module:
task: name=NFCORE_RNASPLICE:RNASPLICE:SUPPA_SALMON:CLUSTEREVENTS_IOE (YRI-GBR); work-dir=/shared/ifbstor1/projects/EPIS_SAV/NF_Core_rnasplice/work/b4/c14bf30ed590b999d1bbc709db90b3
`error [nextflow.exception.MissingFileException]: Missing output file(s)
*.clustvec
expected by process `NFCORE_RNASPLICERNASPLICESUPPA_SALMON:CLUSTEREVENTS_IOE (YRI-GBR)``
Mar-27 15:43:32.298 [TaskFinalizer-4] DEBUG nextflow.processor.TaskProcessor - Handling unexpected condition for
task: name=NFCORE_RNASPLICE:RNASPLICE:SUPPA_SALMON:CLUSTEREVENTS_IOI (GBR-YRI); work-dir=/shared/ifbstor1/projects/EPIS_SAV/NF_Core_rnasplice/work/f5/164864610611480584d75053e7ecb5
`error [nextflow.exception.MissingFileException]: Missing output file(s) `.clustvec`* expected by process `NFCORE_RNASPLICERNASPLICESUPPA_SALMON:CLUSTEREVENTS_IOI (GBR-YRI)``
Mar-27 15:43:34.086 [TaskFinalizer-9] DEBUG nextflow.processor.TaskProcessor - Handling unexpected condition for
task: name=NFCORE_RNASPLICE:RNASPLICE:SUPPA_SALMON:CLUSTEREVENTS_IOI (YRI-GBR); work-dir=/shared/ifbstor1/projects/EPIS_SAV/NF_Core_rnasplice/work/ab/c11527f40c487eda8e8c8fea079629
From the .nextflow.log we can read:
-[nf-core/rnasplice] Pipeline completed with errors-
ERROR ~ Error executing process > 'NFCORE_RNASPLICE:RNASPLICE:SUPPA_SALMON:CLUSTEREVENTS_IOE (GBR-YRI)'
Caused by:
`Missing output file(s)
*.clustvec
expected by process `NFCORE_RNASPLICERNASPLICESUPPA_SALMON:CLUSTEREVENTS_IOE (GBR-YRI)``
Command executed:
suppa.py \
clusterEvents \
--dpsi GBR-YRI_local_diffsplice.dpsi \
--psivec GBR-YRI_local_diffsplice.psivec \
--dpsi-threshold 0.05 \
--eps 0.05 \
--metric euclidean \
--min-pts 20 \
--groups 1-2,3-4 \
--clustering DBSCAN \
-o GBR-YRI_local_cluster
Command output:
Number of clustered events: 0/6 (0.000000%)
Impossible to calculate silhouette score. Only 1 cluster group identified.
The command I run is :
nextflow run nf-core/rnasplice -r 1.0.4 -profile test,ifb_core --outdir ${out_dir}
p
That's an already reported issue. I tried to push a PR to fix the problem, but apparently the current code is not compatible with nextflow 26, so the Github CIs fail. That would require a massive refactor of the workflow code as it heavily uses deprecated syntax. I can work on that but I'd like first to check with the maintainers.