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On each collection, you have to configure which user/users or group/group have access. which user/group is configured on the old collection and which user/group is configured on the new collection?

i have done this already. I then test a user with mstsc to the connection broker hostname and it always redirects to collection 1 although it has only access to collection 2. IF i go from web access then the rdp file is correct and is already prepared for login to collection 2

My guess is that when you connect via mstsc it always connects to the first collection configured, if the user has access to that collection

yes im also think the same

Using the RD Web Access/HTML/RemoteApp and Desktop Connection is the "correct" way to access RDS, as the RD Connection Broker enumerates all collections

Anestis, create a new collection of type "personal unmanaged", you can then assign an individual user to each machine. This is how we emulate vdi with MS RDS technology in Hoster/Cloud scenario where tenant does not have access to the hypervisor. DM me for code snippets, or you can find them on MS docs page.