Hi all! After a long period of absence from nf-core, I finally had two good excuses for working on the nf-core/isoseq pipeline again. First, I have been involved in an Isoseq project which required to update the pipeline. Second, I'm writing a book chapter for RNA Bioinformatics on isoseq analysis with nf-core/isoseq. The project I worked on were on Kinnex Isoseq. The final data is the same, full length non chimeric consenus sequence, but the provided raw data are pre-analyzed and running CCS isn't required. This motivated the implementation of flexible start where the user can start the pipeline at any stage of the isoseq analysis or just map reads and annotate a genome. This have been implemented through a new samplesheet format, where the user can specify the starting stage. I would be grateful to have a code review and some help to understand why the nf-test fails under nextflow 25.10.4. Obviously, I'll be happy to exchange some reviews, even though I didn't done some for a while 😅 github.com/nf-core/isoseq/pull/62