Hello, I have been trying to help get ampliseq working on our HPC, but ran into the error

Downloading plugin nf-validation@1.1.3

ERROR ~ Plugin with id nf-validation not found in any repository

. The automatic downloading doesn't work because the compute nodes do not have internet, so we downloaded nf-validation 1.1.3 using

nextflow plugin install nf-validation@1.1.3

. We're still getting the error message — is there a way to specify the path to the manually installed nf-validation or a specific location where we should move nf-validation?

Not sure why that doesn't work but if the issue is only that the compute nodes lack connection, assuming a shared home directory, is probably to run through the pipeline on the submission/login node with the

test

profile (that should be light enough not to cause complaints except for some very strict setups).

There is an environment variable called

$NXF_PLUGINS_DIR

which you can set to the directory that contains the plugin. (Usually a subdirectory of

$NXF_HOME

, but can be moved elsewhere)

Thanks for the responses! The

NXF_PLUGINS_DIR

variable seems to have solved the issue.