:x: scrnaseq/test_full failed :crying_cat_face: Ru...
# scrnaseq
f
❌ scrnaseq/test_full failed 😿 Run Name romantic_edison Duration 7m 26s Status ❌ Failed Error Message
Copy code
1:54PM INF [seqera-fusion] setting up AWS Batch spot instance monitoring provider=aws-batch
1:54PM INF [seqera-fusion] instance metadata ami-id=ami-0acc5dd6b31082def instance-id=i-092ff780cb05e8743 instance-type=c6id.12xlarge
1:54PM INF [seqera-fusion] starting incremental snapshot timer interval=300000
	STAR --genomeDir STARIndex --readFilesIn pbmc8k_S1_L007_R2_001.fastq.gz,pbmc8k_S1_L008_R2_001.fastq.gz pbmc8k_S1_L007_R1_001.fastq.gz,pbmc8k_S1_L008_R1_001.fastq.gz --runThreadN 12 --outFileNamePrefix pbmc8k. --soloCBwhitelist whitelist.uncompressed.txt --soloType CB_UMI_Simple --soloFeatures Gene --soloUMIlen 10 --sjdbGTFfile genome_genes.gtf --outSAMattrRGline ID:pbmc8k SM:pbmc8k --soloCellFilter CellRanger2.2 10000 0.99 10 --readFilesCommand zcat --runDirPerm All_RWX --outWigType bedGraph --twopassMode Basic --outSAMtype BAM SortedByCoordinate --limitBAMsortRAM 77309411328
	STAR version: 2.7.10b   compiled: 2022-11-01T09:53:26-04:00 :/home/dobin/data/STAR/STARcode/STAR.master/source
May 28 13:54:13 ..... started STAR run
May 28 13:54:14 ..... loading genome
EXITING because of FATAL ERROR: Genome version: 20201 is INCOMPATIBLE with running STAR version: 2.7.10b
SOLUTION: please re-generate genome from scratch with running version of STAR, or with version: 2.7.4a
May 28 13:54:14 ...... FATAL ERROR, exiting
zcat: write error: Broken pipe
pbmc8k._STARtmp//readsCommand_read2: line 4: echo: write error: Broken pipe
zcat: write: Broken pipe
zcat: write error: Broken pipe
zcat: write: Broken pipe
zcat: write error: Broken pipe
zcat: write: Broken pipe
pbmc8k._STARtmp//readsCommand_read1: line 4: echo: write error: Broken pipe
zcat: write error: Broken pipe
zcat: write: Broken pipe
FUSION_GPU_USED=false
Fusion Info:
    available_disk: start:2.8TB, min:2.8TB, max:2.8TB, end:2.8TB
    clone_namespace: false
    download: 2293.56MB, drops:0, skip:0, sync:0, req:19
    fusion_version: 2.5.12-8ea351d
    kernel_version: 6.1
    max_open_files: 1048576
    upload: 11.96MB, dro...
Command Line
Copy code
nextflow run '<https://github.com/nf-core/scrnaseq>' -name romantic_edison -params-file '<https://api.cloud.seqera.io/ephemeral/ar8SLDVM-kSYQinORabCFA.json>' -with-tower -r b7366482866bb64cf25a8c46940af2ab363698af -profile test_full
May 28, 2026 at 1:54 PM