Luca Cozzuto
04/01/2026, 10:57 AMERROR Module 'vsearch/cluster' not found in available modules
how can I fix this? Can someone help me, please :)mashehu
04/01/2026, 10:59 AMLuca Cozzuto
04/01/2026, 11:00 AMnf-core modules install vsearch/clustermashehu
04/01/2026, 11:18 AMnf-core --verbose m install vsearch/cluster?Luca Cozzuto
04/01/2026, 11:36 AMnf-core --verbose m install vsearch/cluster
,--./,-.
___ __ __ __ ___ /,-._.--~\
|\ | |__ __ / ` / \ |__) |__ } {
| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'
nf-core/tools version 3.5.2 - <https://nf-co.re>
DEBUG sys.platform='linux', git_executable='git' util.py:494
Pulling from 'nf-core/modules' (<https://github.com/nf-core/modules.git>) ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ Waiting for responseDEBUG Popen(['git', 'version'], cwd=/tmp/.config/nfcore/nf-core/modules, stdin=None, shell=False, universal_newlines=False) cmd.py:1270
DEBUG Popen(['git', 'fetch', '-v', '--progress', '--', 'origin'], cwd=/tmp/.config/nfcore/nf-core/modules, stdin=None, shell=False, cmd.py:1270
universal_newlines=True)
DEBUG Popen(['git', 'checkout', 'master'], cwd=/tmp/.config/nfcore/nf-core/modules, stdin=None, shell=False, universal_newlines=False) cmd.py:1270
DEBUG Popen(['git', 'merge', 'origin/master'], cwd=/tmp/.config/nfcore/nf-core/modules, stdin=None, shell=False, universal_newlines=False) cmd.py:1270
DEBUG Got '/tmp/.config/nfcore/nf-core/modules' as path utils.py:369
DEBUG Found a config cache, loading: /tmp/.nextflow/nf-core/wf-config-cache-9f4bce35ba6f9f759cbf54fa7.json utils.py:403
DEBUG Using config file: /tmp/.config/nfcore/nf-core/modules/.nf-core.yml utils.py:1501
DEBUG Got '.' as path utils.py:369
DEBUG Found a config cache, loading: /tmp/.nextflow/nf-core/wf-config-cache-636044805f5ccb3572fc02196.json utils.py:403
DEBUG Using config file: .nf-core.yml utils.py:1501
DEBUG sys.platform='linux', git_executable='git' util.py:494
DEBUG Popen(['git', 'checkout', 'master'], cwd=/tmp/.config/nfcore/nf-core/modules, stdin=None, shell=False, universal_newlines=False) cmd.py:1270
DEBUG Popen(['git', 'merge', 'origin/master'], cwd=/tmp/.config/nfcore/nf-core/modules, stdin=None, shell=False, universal_newlines=False) cmd.py:1270
DEBUG Got '/tmp/.config/nfcore/nf-core/modules' as path utils.py:369
DEBUG Found a config cache, loading: /tmp/.nextflow/nf-core/wf-config-cache-9f4bce35ba6f9f759cbf54fa7.json utils.py:403
DEBUG Using config file: /tmp/.config/nfcore/nf-core/modules/.nf-core.yml utils.py:1501
WARNING There was an error running the prettier pre-commit hook. lint_utils.py:172
STDOUT: [INFO] Installing environment for <https://github.com/pre-commit/mirrors-prettier>.
[INFO] Once installed this environment will be reused.
[INFO] This may take a few minutes...
An unexpected error has occurred: AssertionError:
Check the log at /tmp/.cache/pre-commit/pre-commit.log
STDERR:
DEBUG Popen(['git', 'checkout', 'master'], cwd=/tmp/.config/nfcore/nf-core/modules, stdin=None, shell=False, universal_newlines=False) cmd.py:1270
ERROR Module 'vsearch/cluster' not found in available modules install.py:236
DEBUG entering code: StateBlock(line=0,level=0,tokens=0), 0, 1, False code.py:11
DEBUG entering fence: StateBlock(line=0,level=0,tokens=0), 0, 1, False fence.py:10
DEBUG entering blockquote: StateBlock(line=0,level=0,tokens=0), 0, 1, False blockquote.py:13
DEBUG entering hr: StateBlock(line=0,level=0,tokens=0), 0, 1, False hr.py:15
DEBUG entering list: StateBlock(line=0,level=0,tokens=0), 0, 1, False list.py:102
DEBUG entering reference: StateBlock(line=0,level=0,tokens=0), 0, 1, False reference.py:10
DEBUG entering html_block: StateBlock(line=0,level=0,tokens=0), 0, 1, False html_block.py:35
DEBUG entering heading: StateBlock(line=0,level=0,tokens=0), 0, 1, False heading.py:14
DEBUG entering lheading: StateBlock(line=0,level=0,tokens=0), 0, 1, False lheading.py:10
DEBUG entering paragraph: StateBlock(line=0,level=0,tokens=0), 0, 1, False paragraph.py:11
╭─ info ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────╮
│ │
│ Use the command nf-core modules list to view available modules. │
│ │
╰──────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────╯
ERROR __main__.py:91mashehu
04/01/2026, 11:39 AMLuca Cozzuto
04/01/2026, 11:40 AMmashehu
04/01/2026, 11:41 AMLuca Cozzuto
04/01/2026, 11:42 AMLuca Cozzuto
04/01/2026, 11:42 AMmashehu
04/01/2026, 11:44 AM/tmp/.cache/pre-commit/pre-commit.log i am curious if it is indeed just a red herringLuca Cozzuto
04/01/2026, 11:44 AM### version information
pre-commit version: 4.5.1
git --version: git version 2.52.0
sys.version:
3.14.2 | packaged by conda-forge | (main, Jan 26 2026, 195600) [GCC 14.3.0]
sys.executable: /usr/local/bin/python3.14
os.name: posix
sys.platform: linux
### error information
An unexpected error has occurred: AssertionError:
```
Traceback (most recent call last):
File "/usr/local/lib/python3.14/site-packages/pre_commit/error_handler.py", line 73, in error_handler
yield
File "/usr/local/lib/python3.14/site-packages/pre_commit/main.py", line 429, in main
return run(args.config, store, args)
File "/usr/local/lib/python3.14/site-packages/pre_commit/commands/run.py", line 443, in run
install_hook_envs(to_install, store)
~~~~~~~~~~~~~~~~~^^^^^^^^^^^^^^^^^^^
File "/usr/local/lib/python3.14/site-packages/pre_commit/repository.py", line 229, in install_hook_envs
_hook_install(hook)
~~~~~~~~~~~~~^^^^^^
File "/usr/local/lib/python3.14/site-packages/pre_commit/repository.py", line 85, in _hook_install
lang.install_environment(
~~~~~~~~~~~~~~~~~~~~~~~~^
hook.prefix, hook.language_version, hook.additional_dependencies,
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
)
^
File "/usr/local/lib/python3.14/site-packages/pre_commit/languages/node.py", line 80, in install_environment
assert prefix.exists('package.json')
~~~~~~~~~~~~~^^^^^^^^^^^^^^^^
AssertionError
```mashehu
04/01/2026, 11:46 AMmashehu
04/01/2026, 11:46 AMmashehu
04/01/2026, 11:46 AMnf-core modules list remote and search for it?Luca Cozzuto
04/01/2026, 11:47 AMLuca Cozzuto
04/01/2026, 11:47 AMLuca Cozzuto
04/01/2026, 11:47 AMmashehu
04/01/2026, 11:49 AM"vsearch/cluster": {
"branch": "master",
"git_sha": "694b2cdc2d695926dc10ac149e59b7b5b563cef9",
with nf-core/tools 3.5.2mashehu
04/01/2026, 11:50 AMLuca Cozzuto
04/01/2026, 11:52 AMls $HOME/.config/nf-core/cache_3.6.9/
github_info.sqlitemashehu
04/01/2026, 11:52 AMmashehu
04/01/2026, 11:53 AM/tmp/.config/nfcore/nf-core/modules/Luca Cozzuto
04/01/2026, 11:53 AMls /tmp/.config/nfcore/nf-core/modules/modules/nf-core/vsearch/
cluster dereplicate fastqfilter sintax sort usearchglobalLuca Cozzuto
04/01/2026, 11:53 AMmashehu
04/01/2026, 11:54 AMmashehu
04/01/2026, 11:55 AMmodules.json look like?Luca Cozzuto
04/01/2026, 11:57 AM{
"name": "",
"homePage": "",
"repos": {
"<https://github.com/nf-core/modules.git>": {
"modules": {
"nf-core": {
"cat/fastq": {
"branch": "master",
"git_sha": "5d28ea7cfaebcfae6f74d1067ea766d8ff19dff3",
"installed_by": [
"modules"
]
},
"cutadapt": {
"branch": "master",
"git_sha": "624977dfaf562211e68a8a868ca80acc8461f1ac",
"installed_by": [
"modules"
]
},
"fastqc": {
"branch": "master",
"git_sha": "79b36b51048048374b642289bfe9e591ef56fe05",
"installed_by": [
"modules"
]
},
"minimap2/align": {
"branch": "master",
"git_sha": "5c9f8d5b7671237c906abadc9ff732b301ca15ca",
"installed_by": [
"modules"
]
},
"minimap2/index": {
"branch": "master",
"git_sha": "14980f759266eec42dac401fcafeb83d6c957b41",
"installed_by": [
"modules"
]
},
"multiqc": {
"branch": "master",
"git_sha": "575e1a4b51a9bad7a8cd1316a88fb85684ef7c7b",
"installed_by": [
"modules"
]
},
"nanoq": {
"branch": "master",
"git_sha": "4dd9d8439a429c7ee566e0e2347f76ddeef27e66",
"installed_by": [
"modules"
]
},
"samtools/cat": {
"branch": "master",
"git_sha": "f9edc59be2fe25bb6fc73ca4dfc0d28246f2a2d6",
"installed_by": [
"modules"
]
},
"samtools/faidx": {
"branch": "master",
"git_sha": "b2e78932ef01165fd85829513eaca29eff8e640a",
"installed_by": [
"modules"
]
},
"samtools/fastq": {
"branch": "master",
"git_sha": "1d2fbdcbca677bbe8da0f9d0d2bb7c02f2cab1c9",
"installed_by": [
"modules"
]
},
"samtools/sort": {
"branch": "master",
"git_sha": "5cb9a8694da0a0e550921636bb60bc8c56445fd7",
"installed_by": [
"modules"
]
},
"seqkit/seq": {
"branch": "master",
"git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
"installed_by": [
"modules"
]
}
}
},
"subworkflows": {
"nf-core": {
"utils_nfcore_pipeline": {
"branch": "master",
"git_sha": "65f5e638d901a51534c68fd5c1c19e8112fb4df1",
"installed_by": [
"subworkflows"
]
}
}
}
}
}
}mashehu
04/01/2026, 12:01 PMmashehu
04/01/2026, 12:02 PMLuca Cozzuto
04/01/2026, 12:02 PMLuca Cozzuto
04/01/2026, 12:03 PMLuca Cozzuto
04/01/2026, 12:09 PMmashehu
04/01/2026, 12:09 PMpip install --upgrade --force-reinstall git+<https://github.com/nf-core/tools.git@dev>Luca Cozzuto
04/01/2026, 12:18 PMmashehu
04/01/2026, 12:19 PMpipx install git+<https://github.com/nf-core/tools.git@dev> then 🙂Luca Cozzuto
04/01/2026, 12:21 PMLuca Cozzuto
04/01/2026, 12:22 PMpipx install --force git+<https://github.com/nf-core/tools.git@dev>
ERROR: Package 'nf-core' requires a different Python: 3.9.25 not in '<4,>=3.10'
Cannot determine package name from spec 'git+<https://github.com/nf-core/tools.git@dev>'. Check package spec for errors.
lcozzuto in 🌐 biocore in RNAseq3 on v3.0 [!?] on ☁️ (eu-central-1) took 5s
❯ python --version
Python 3.9.25mashehu
04/01/2026, 12:22 PMLuca Cozzuto
04/01/2026, 12:22 PMLuca Cozzuto
04/01/2026, 12:22 PMLuca Cozzuto
04/01/2026, 12:30 PMLuca Cozzuto
04/01/2026, 12:31 PMLuca Cozzuto
04/01/2026, 12:31 PMLuca Cozzuto
04/01/2026, 12:31 PMmashehu
04/01/2026, 12:31 PMnf-core m install?Luca Cozzuto
04/01/2026, 12:35 PMLuca Cozzuto
04/01/2026, 12:35 PMLuca Cozzuto
04/01/2026, 12:35 PMLuca Cozzuto
04/01/2026, 12:35 PMLuca Cozzuto
04/01/2026, 12:35 PMLuca Cozzuto
04/01/2026, 12:36 PMmashehu
04/01/2026, 12:38 PMLuca Cozzuto
04/01/2026, 12:46 PM