Hi all! I wanted to share a pipeline I’ve been working on called KOCheck ✅. KOCheck ✅ is a workflow for validating genetic knockouts from sequencing data. It’s designed primarily for microbial genetics workflows using short reads and performs QC, alignment, coverage analysis, marker/deletion validation, and summary report writing to facilitate quick interpretation. If anyone has suggestions for improving the pipeline structure, especially since this is my first foray into Nextflow, I’d really appreciate feedback! (More details below in the 🧵) Thanks! Repo: https://github.com/meadm/KOCheck