Hi, I’m running into an issue when running the nf-core/metatdenovo pipeline (v1.3.0) on a cluster. I’m annotating metatranscriptomic data with eggNOG, and the eggNOG database is already available on the cluster. I’ve specified the database path in my nf-params.json file using: "eggnog_dbpath": "/sw/data/eggNOG_data/5.0.0/rackham" However, the pipeline seems to ignore this parameter and instead tries to download the database from http://eggnogdb.embl.de/download/emapperdb-5.0.2/, which fails with a 404 error and interrupts the run. Has anyone encountered this before, or does anyone have suggestions for how to force the pipeline to use the local eggNOG database instead of attempting a download?

Hi, @Ebba Perman! Slack uses channels to organise discussions. Press the

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more option on the left sidebar. Then hover over Your organisation, and finally select Channels to browse. Each nf-core pipeline has it’s own channel (e.g. #CE8SSJV3N). Please post any questions about specific pipelines in the relevant channel. If you’re not sure where to post your message, please ask @James Fellows Yates for help.

#C02FC6VFQG1 is the bestplace to ask as it's a pipeline specific question

Can you show how you run this pipeline along with a

ls

of that directory?