High guys. Still running into issues. Struggling f...
# rnaseq
u
High guys. Still running into issues. Struggling for the whole day and really need some help.
--profile docker
--aligner star_salmon
-c custom.config
After TRIMGALORE, STAR_ALIGN_IGENOMES is not working. exit status:139/Command error:Segmentation fault repeated I tried set the resource limit 12 cpus/80Gb RAM and failed I tried local path for the STAR_index-> failed at STAR_ALIGN_IGENOMES, segfault I tried removed
--genome GRCh38
and use
--fasta --gtf
to build the index -> failed at STAR_GENOMEGENERATE_IGENOMES, segfault Not really knowing why these error occurred repeatly. I have checked memory for docker/WSL and also monitor the RAM during the process, it should be enough In previous discussion, segfault is associated with RAM allocation or restriction, which i did not know how to fix. I also search on the internet, conversation in the slack, issues on the github. Still cannot find the solution. Really sorry to bother you. Since I just learned python and coding for three months, there is still a lot to learn. Any suggestion might be helpful. Thank you.