Jacob Egemose-Høgfeldt
09/25/2025, 2:04 PMJacob Egemose-Høgfeldt
09/25/2025, 2:06 PMnextflow run nf-core/airrflow -r 4.3.0 \
-profile clontech_umi_bcr,singularity \
--input /x/x/x/x/x/Light_Chain.tsv \
-c /x/x/x/x/resources.config \
--outdir /x/x/x/x/x/AIRRFLOW_results \
-resume \
--clonal_threshold 0.15
And for some reason I have to add the --clonal_threshold 0.15 for the lightchain sequencing data, otherwise it complains that it cant auto calulate this.
I have no issues running the same analysis on the Heavy-Chain sequencing data (however for that I run without the --clonal_threshold parameter)