Hi, I am trying to run AIRRFLOW on bulk fastq.gz f...
# airrflow
j
Hi, I am trying to run AIRRFLOW on bulk fastq.gz files of light-chain sequences (without heavy-chains). I get an error saying that I need the column "cell_id". By looking further, I can see 'cell_id' column is not mentioned in the bulk usage, however it is mentioned in the single cell usage. How do I fix this issue? My inputsample file looks like this: Let me know if you need further information, thank you
This is the command I run for the analysis:
nextflow run nf-core/airrflow -r 4.3.0 \
-profile clontech_umi_bcr,singularity \
--input /x/x/x/x/x/Light_Chain.tsv \
-c /x/x/x/x/resources.config \
--outdir /x/x/x/x/x/AIRRFLOW_results \
-resume \
--clonal_threshold 0.15
And for some reason I have to add the --clonal_threshold 0.15 for the lightchain sequencing data, otherwise it complains that it cant auto calulate this. I have no issues running the same analysis on the Heavy-Chain sequencing data (however for that I run without the --clonal_threshold parameter)