Jeremy Simon
09/18/2025, 2:40 PMsimpleaf to allow processing of 10x 5' v2 chemistry?
It is described here https://simpleaf.readthedocs.io/en/stable/quant-command.html under
-d, --expected-ori <EXPECTED_ORI>
The expected direction/orientation of alignments in the chemistry being processed.
If not provided, will default to `fw` for 10xv2/10xv3, otherwise `both` [possible values: fw, rc, both]
and is important to get this right for 3' vs 5' chemistry as noted in my issue here https://github.com/COMBINE-lab/alevin-fry/issues/118 and discussed elsewhere. I don't see this listed in any of the nf-core/scrna docs but perhaps could be passed manually in a custom yaml?