Hi all, perhaps a question for <@U01GQAH4X1N> but ...
# scrnaseq
j
Hi all, perhaps a question for @Rob Patro but is there a way to specify the expected orientation for
simpleaf
to allow processing of 10x 5' v2 chemistry? It is described here https://simpleaf.readthedocs.io/en/stable/quant-command.html under
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-d, --expected-ori <EXPECTED_ORI>      
The expected direction/orientation of alignments in the chemistry being processed. 
If not provided, will default to `fw` for 10xv2/10xv3, otherwise `both` [possible values: fw, rc, both]
and is important to get this right for 3' vs 5' chemistry as noted in my issue here https://github.com/COMBINE-lab/alevin-fry/issues/118 and discussed elsewhere. I don't see this listed in any of the
nf-core/scrna
docs but perhaps could be passed manually in a custom yaml?