Hello, everyone! Thank you for this amazing tool. I was wondering if anyone has run this pipeline for DNA instead of RNAseq? In our case, we have a library that contains B-cell receptor sequences from the variable regions to the J chain of the heavy, kappa and lambda chains that were created using several PCR steps and then sequenced. Since this is DNA, the C chain is not included. Does anyone know if it would still be possible to run the pipeline with this kind of data? help is greatly appreciated 🙂

Hi Ana, yes it is possible to run it with J region primers instead of C region primers. You can just provide them with the --c_primers parameter instead. Feel free to ask here if you have any questions on how to set up the parameters for your custom protocol!

Hello, Gisela: Thank you for your prompt response. Great, I will try it like this then. Thank you very much! 🙂