Hi there, I am running `nf-core/crisprseq -r 2.2.0...
# crisprseq
a
Hi there, I am running
nf-core/crisprseq -r 2.2.0 --analysis screening
and get a failure to pull the singularity image for mageckflute-2.2.0. It looks here: https://depot.galaxyproject.org/singularity/mageckflute:2.2.0--r42hdfd78af_0, but the closest thing in the repository is prefixed with bioconductor:
Copy code
bioconductor-mageckflute:2.2.0--r42hdfd78af_0
Does anyone know a fix for this? Command in full:
Copy code
nextflow run nf-core/crisprseq -r 2.2.0 --analysis screening --input samplesheet.csv --library brunello_library.txt --outdir results_screen --contrasts contrasts.txt  -profile singularity
Error in full:
Copy code
ERROR ~ Error executing process > 'NFCORE_CRISPRSEQ:CRISPRSEQ_SCREENING:MAGECK_FLUTEMLE_CONTRASTS (1)'

Caused by:
  Failed to pull singularity image
  command: singularity pull  --name depot.galaxyproject.org-singularity-mageckflute-2.2.0--r42hdfd78af_0.img.pulling.1719581400961 <https://depot.galaxyproject.org/singularity/mageckflute:2.2.0--r42hdfd78af_0> > /dev/null
  status : 255
  message:
    INFO:    Downloading network image
    FATAL:   the requested image was not found



 -- Check '.nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: <https://nf-co.re/docs/usage/troubleshooting>

 -- Check '.nextflow.log' file for details
-[nf-core/crisprseq] Pipeline completed with errors-
l
Hi Adam, good catch thank you, seems to be a typo/bug. I will fix this on the dev branch
a
Great. Thanks @Laurence Kuhlburger!
l
hi @Adam Reid any chance you could try on my dev branch just to see if it works? https://github.com/LaurenceKuhl/crisprseq/tree/dev
a
OK, running it now...
That worked great! Thanks Laurence.
🙌 1
j
facing the same issue on my end. is there an easy way to execute from your dev branch or do i have to clone the repo etc? or alternatively, do you have an expected timeline for this to be patched into v2.2.1?
j
Hi @Jeremy Simon, @Laurence Kuhlburger fixed this on the dev version new. You can run it with
nextflow run nf-core/crisprseq -r dev ...
until we don’t have the new v2.2.1
🙌 1