Hello! with a colleague we are trying to run metatdenovo on 5 samples focusing on bacteria. I just got this error message. Should I just download gtdb and eukulele by myself or is there a simpler way? I also join our params.yml. Thank you 🙂

Hello @Thomas Mollica! could you check in

/home/evalopes/metadnovo23/work/a3/a8bdf174c51d0bff6e74af937117b7

if you have a diamond file and if it is not empty? (so if it has any hits).

Hi @Danilo Di Leo! With @Eva Lopes - PhD student I am helping with this - we checked the work folder and we only have path_test.csv and free.csv files. I was thinking to give a shot to DIAMOND, which one do you think would work best for prokaryotic data?

The best for prokaryotes is still gtdb database https://figshare.scilifelab.se/articles/dataset/nf-core_metatdenovo_taxonomy/28211678

Ok, I will download this gtdb made for Diamond. Thanks for providing all of this! Do you have an idea why Eukeulele failed?

It could be that the tool didn't find any annotation and the output was empty. But also there are hiccups with eukulele so there might be more.