A new request to upload a dataset to nf-core AWS S3 bucket has been made! • Requested by: @Daniel Lundin • Pipeline name: metatdenovo • Why is this needed?: Databases for annotation. I'm actually not certain what makes sense in tests. The Kofamscan database is the most important and the one we use in our projects for functional annotation. The dbCAN is less important. The only functional annotation done by the full scale tests today is the quite outdated eggNOG-mapper against v. 5 of the eggNOG database. I think we chose this because it was already uploaded. 1. dbCAN to

<s3://ngi-igenomes/test-data/metatdenovo/dbcan/dbcan_db/>

. The last full test downloaded it but couldn't write it there (403). A copy should still be in

<s3://nf-core-awsmegatests/work/metatdenovo/work-dev/9d/a7d3c1b25a8fc0407b25e0bf458351/dbcan_db/>

(about 5–6 GB); otherwise

run_dbcan database --no-cgc --db_dir dbcan_db --aws_s3

(dbCAN 5.2.9) recreates it. 2. KOfamScan to `s3://ngi-igenomes/test-data/metatdenovo/kofam/`:

ko_list

(unzipped from https://www.genome.jp/ftp/db/kofam/ko_list.gz) and the

profiles/

directory (unpacked from https://www.genome.jp/ftp/db/kofam/profiles.tar.gz). • Size of the dataset: 14 and 8 GB respectively • URL: See instructions above. • Related files: Core team will add this dataset to the nf-core bucket. Please react to the message with ✅ when this is done. [This process is not automated]