Could I clarify if star_salmon and salmon (alignment free) both filter out reads of unexpected lib types? E.g for a reverse stranded library, it removes forward/unstranded reads?
j
Jonathan Manning
09/28/2026, 8:26 AM
No, there's not explicit filtering, detected or specified strandedness is just passed to the quantification tools such as Salmon, which likely amounts to the same thing but you should satisfy yourself there.