:x: sarek/test_full_germline_aws failed :crying_ca...
# sarek_dev
f
❌ sarek/test_full_germline_aws failed 😿 Run Name sarek_test_full_germline_aws Duration 4h 13m 23s Status ❌ Failed Error Message
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19:11:19.626 INFO  HaplotypeCaller - The Genome Analysis Toolkit (GATK) v4.6.2.0
19:11:19.626 INFO  HaplotypeCaller - For support and documentation go to <https://software.broadinstitute.org/gatk/>
19:11:19.626 INFO  HaplotypeCaller - Executing as root@ip-172-31-15-185.eu-west-1.compute.internal on Linux v6.1.166-197.305.amzn2023.x86_64 amd64
19:11:19.627 INFO  HaplotypeCaller - Java runtime: OpenJDK 64-Bit Server VM v17.0.11-internal+0-adhoc..src
19:11:19.627 INFO  HaplotypeCaller - Start Date/Time: August 7, 2026 at 7:11:19 PM GMT
19:11:19.627 INFO  HaplotypeCaller - ------------------------------------------------------------
19:11:19.627 INFO  HaplotypeCaller - ------------------------------------------------------------
19:11:19.628 INFO  HaplotypeCaller - HTSJDK Version: 4.2.0
19:11:19.629 INFO  HaplotypeCaller - Picard Version: 3.4.0
19:11:19.629 INFO  HaplotypeCaller - Built for Spark Version: 3.5.0
19:11:19.631 INFO  HaplotypeCaller - HTSJDK Defaults.COMPRESSION_LEVEL : 2
19:11:19.632 INFO  HaplotypeCaller - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false
19:11:19.632 INFO  HaplotypeCaller - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true
19:11:19.632 INFO  HaplotypeCaller - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false
19:11:19.633 INFO  HaplotypeCaller - Deflater: IntelDeflater
19:11:19.633 INFO  HaplotypeCaller - Inflater: IntelInflater
19:11:19.633 INFO  HaplotypeCaller - GCS max retries/reopens: 20
19:11:19.633 INFO  HaplotypeCaller - Requester pays: disabled
19:11:19.633 INFO  HaplotypeCaller - Initializing engine
19:11:24.512 INFO  HaplotypeCaller - Shutting down engine
[August 7, 2026 at 7:11:24 PM GMT] org.broadinstitute.hellbender.tools.walkers.haplotypecaller.HaplotypeCaller done. Elapsed time: 0.09 minutes.
Runtime.totalMemory()=201326592
java.lang.RuntimeException: CRAM version 3.1 is not supported
	at htsjdk.samtools.cram.build.CramIO.readCramHeader(CramIO.java:132)
	at htsjdk.samtools.cram.build.CramConta...
Command Line
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nextflow run '<https://github.com/nf-core/sarek>' -name sarek_test_full_germline_aws -params-file '<https://api.cloud.seqera.io/ephemeral/bM2hh-3_be4G1Avsg9vLgA.json>' -with-tower -r dev -profile test_full_germline_aws
Aug 7, 2026 at 7:11 PM