Hi! I am trying to use the scrnaseq pipeline but I am getting an error when connecting to iGenome. I have tried to ignore igenomes but the error persists. Command:

NXF_VER=25.10.4 nextflow run nf-core/scrnaseq \
    -profile test \
    --aligner cellranger \
    --fasta ~/Shared-MarcelCosta/apps/cellranger-10.0.0/refdata-gex-GRCh38-2024-A/fasta/genome.fa \
    --cellranger_index ~/Shared-MarcelCosta/apps/cellranger-10.0.0/refdata-gex-GRCh38-2024-A/fasta/genome.fa.fai \
    --gtf ~/Shared-MarcelCosta/apps/cellranger-10.0.0/refdata-gex-GRCh38-2024-A/genes/genes.gtf.gz \
    --outdir PROCESSING
    --genome null \
    --igenomes_ignore

Error:

* --igenomes_base (<s3://ngi-igenomes/igenomes/>): could not validate file format of '<s3://ngi-igenomes/igenomes/>': Access Denied (Service: S3, Status Code: 403, Request ID: 4F61R9KM8W39WSMT, Extended Request ID: qa4D4BHgJMfa2KLuSmPYARTuZnKR/ObNHgeSSM6ur226/3vZVhr/BYGvg77an3ssxHERDxzWFZ20SBSWl1oofjWAUIeRrTvz) (SDK Attempt Count: 1)

I don't need the iGenomes access because I already have the genome in local.

You should be able to point

igenones_base

at your local copy?

Yes! Thanks! Specifying igenomes_base worked.