Lech Kaczmarczyk
08/26/2024, 3:31 PMERROR ~ Cannot get property 'baseName' on null object
-- Check script '/cfs/klemming/projects/snic/sllstore2017078/lech/sarek/sarek/./workflows/sarek/../../subworkflows/local/bam_variant_calling_germline_all/main.nf' at line: 133 or see '.nextflow.log' file for more details
ERROR ~ Cannot get property 'baseName' on null object
-- Check script '/cfs/klemming/projects/snic/sllstore2017078/lech/sarek/sarek/./workflows/sarek/../../subworkflows/local/bam_variant_calling_germline_all/main.nf' at line: 134 or see '.nextflow.log' file for more details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: <https://nf-co.re/docs/usage/troubleshooting>
-- Check '.nextflow.log' file for details
referred lines 133-135:
dbsnp.map{ it -> [[id:it[0].baseName], it] },
dbsnp_tbi.map{ it -> [[id:it[0].baseName], it] },
intervals)
my command:
nextflow run /cfs/klemming/projects/snic/sllstore2017078/lech/sarek/sarek \
-profile pdc_kth \
-name $1 \
-params-file /cfs/klemming/projects/snic/sllstore2017078/lech/sarek/$2 \
--project=${COMPUTE_ACCOUNT} \
--email=${USER_E_MAIL} \
--step prepare_recalibration \
--known_snps /cfs/klemming/projects/snic/sllstore2017078/lech/sarek/run1_no_GATK/get_known_sites_vcf/id_04.germline.pass.intersected.vcf.gz \
--known_snps_tbi /cfs/klemming/projects/snic/sllstore2017078/lech/sarek/run1_no_GATK/get_known_sites_vcf/id_04.germline.pass.intersected.vcf.gz.tbi
json:
{
"input": "/cfs/klemming/projects/snic/sllstore2017078/lech/sarek/sample_tables/atac_bams_for_sarek_1samp.csv",
"outdir": "/cfs/klemming/projects/supr/sllstore2017078/lech/sarek/ail_recal_test1",
"genome": "",
"no_intervals": false,
"tools": "deepvariant,freebayes,strelka,haplotypecaller",
"concatenate_vcfs": true,
"joint_germline": true,
"save_output_as_bam": true,
"known_indels_vqsr": false,
"known_snps_vqsr": false,
"ngscheckmate_bed": false,
"use_gatk_spark": "baserecalibrator",
"fasta": "/cfs/klemming/projects/snic/sllstore2017078/lech/sarek/refs/112/genome/Gallus_gallus.bGalGal1.mat.broiler.GRCg7b.dna_sm.toplevel.fa",
"igenomes_ignore": true
}Rike Hanssen
08/26/2024, 3:33 PMLech Kaczmarczyk
08/26/2024, 3:42 PMRike Hanssen
08/26/2024, 3:43 PMLech Kaczmarczyk
08/26/2024, 3:53 PMRike Hanssen
08/26/2024, 3:54 PM-r 3.4.3 it should make sure you are on a fixed revision. it is also reported in the multiqc report, the execution htmls and the .nextflow.log on startupLech Kaczmarczyk
08/26/2024, 3:59 PMRike Hanssen
08/26/2024, 4:00 PMLech Kaczmarczyk
08/27/2024, 11:15 AM