Hi everyone, I’m working on an RNA-Seq project in a non-profit research setting and I’m running into some challenges with running the NFCore RNA-Seq pipeline due to limited computational resources. I was wondering if anyone has experience running the pipeline without access to a high-end computer? I’m particularly interested in solutions that are efficient, easy to integrate with NFCore, and cost-effective—like cloud services, lightweight alternatives, or other workarounds. Any advice or shared experiences would be greatly appreciated! Thanks so much in advance.

Hi! Some cloud providers provide free trial credits for new users. Maybe you could take advantage of that. For example, Google gives 300$ for new users. Hope it helps :)

See also https://nf-co.re/blog/2025/nf-core-colab-guide

If you know someone in the United States you can work with, have a look at https://access-ci.org/ and see if that would work for you. If you can get access cloud computing or an HPC, I would definitely recommend using that, but in principle you could try get an older server for cheap and use that, 64 GB RAM should suffice for #CE8SSJV3N, and more CPUs will be faster, but if you have time, you can probably get by even with one ...