Hi, Im running methylseq 4.2.0 on an EM-seq datase...
# methylseq
a
Hi, Im running methylseq 4.2.0 on an EM-seq dataset. The pipeline keep exiting on the alignment process (NFCORE_METHYLSEQMETHYLSEQFASTQ_ALIGN_DEDUP_BISMARK:BISMARK_ALIGN) with the error exit status 140:
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Feb-09 06:07:58.390 [TaskFinalizer-2] DEBUG nextflow.processor.TaskProcessor - Handling unexpected condition for
  task: name=NFCORE_METHYLSEQ:METHYLSEQ:FASTQ_ALIGN_DEDUP_BISMARK:BISMARK_ALIGN (YD-4288-4); work-dir=/crex/proj/EMseq/work/61/cf56171fa792dea0289b278cd58aa5
  error [nextflow.exception.ProcessFailedException]: Process `NFCORE_METHYLSEQ:METHYLSEQ:FASTQ_ALIGN_DEDUP_BISMARK:BISMARK_ALIGN (YD-4288-4)` terminated with an error exit status (140)
The fastq files are quite big, ~6Gb * 6 (re-sequenced x 6) * 2 (paired-end) = 70-80Gb. Is this a memory issue? Or something else? Any ideas?
n
exitcode
140
usually means your job ran out of time
a
cheers! I’ll try that