Hi All, What is the recommended sequencing depth, read length, and library layout (se/pe) for: (1) TF (FOXA2), (2) broad histone mods (h3k27me3), and (3) narrow (H3k4me3)? Chat GPT gave me the attached table, but I would really appreciate getting some human feedback. Thanks!!

Sounds about right-ish to me. Altough I think the number of reads doesn't really matter more than the actual signal/noise ratio, above a certain depth. About the read length it depends on weither you use se or pe, pe will probably be more fitted for histone modifications because you'll be more accurate on fragment length. Finally the number of replicates depends on what you can afford, the more is the best IMO.