Ting Yang
02/06/2026, 4:44 PMexport TMPDIR=${tmp}/temp_F1_Sarek
NXF_OPTS='-Xms1g -Xmx4g'
nextflow run nf-core/sarek -r 3.7.0 -profile conda \
--input ./samplesheet.csv --step mapping --outdir ./results \
--aligner bwa-mem2 --genome GATK.GRCh38 \
--tools mutect2,strelka -c nextflow.config -with-report --dbsnp ${database}/GRCh38/resources_broad_hg38_v0_Homo_sapiens_assembly38.dbsnp138.vcf --known_indels ${database}/GRCh38/Homo_sapiens_assembly38.known_indels.vcf.gz --pon ${database}/GRCh38/PON.sorted.vcf.gz --pon_tbi${database}/GRCh38/PON.sorted.vcf.gz.tbi --germline_resource${database}/GRCh38/af-only-gnomad.hg38.vcf.gz --wes --intervals ${database}/GRCh38/GRCh38_exome.bed --only_paired_variant_calling
I wonder if anyone experience sth similar, thanks!