I am getting this error on the latest release of the pipeline (I also posted this to Github, issue 567):
Command exit status:
1
Command output:
ERROR: failed to parse CSV at line: 1, col: 1
This is my command:
nextflow run ./scrnaseq/main.nf -profile singularity,gpu \
--input samplesheet.csv \
--protocol auto \
--genome GRCh38 \
--aligner cellrangermulti \
--outdir outdir \
--gtf gencode.v49.annotation.gtf \
--cellranger_multi_barcodes multi_barcodes.csv \
--gex_target_panel Chromium_Human_Transcriptome_Probe_Set_v2.0.0_GRCh38-2024-A.csv \
--gex_reference_version GRCh38 \
--skip_cellrangermulti_vdjref \
-resume
I suspect there is an issue with script in creating the proper config file for cellrangermulti: scrnaseq/modules/nf-core/cellranger/multi/templates/cellranger_multi.py