hello! Could you confirm me that before 3.23, UMIs were NOT taken into account by the alignment/quantification pipeline, therefore the UMI step in post-processing was "only" for QC?
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Matthias Zepper
05/16/2026, 10:31 PM
Where did you get this info from? UMI support was added gradually and e.g. a position in R2 was for a while not yet supported, but latest ~3.9 ish should have had all the features for a proper deduplication using UMIs in place.