Hi, I am getting a strange error:

The following invalid input values have been detected:

* --skip_dada_addspecies (true): Value is [string] but should be [boolean]

but my command line is simply

--skip-dada-addspecies

without any value. I tried putting the parameter in several places of the command line but I always get the same error. Any tips on how to solve this? The complete command line is:

nextflow run nf-core/ampliseq -profile singularity -resume --input "samplesheet.csv" --FW_primer "TGCGGTATTWTAACTGTACT" --RV_primer "TTATTCCTTRATCACCC" --dada_ref_tax_custom "MIDORI2_UNIQ_NUC_GB269_lrRNA_DADA2.fasta.gz" --dada_assign_taxlevels "Class,Subclass,Order,Family,Genus,Species" --exclude_taxa "none" --outdir results --skip_dada_addspecies

Hi there, thats a new error to me. What version of the pipeline is that? (you can fix the version with e.g.

-r 2.17.0

) Also, the nextflow version might be important here, what is it? (You can fix the nextflow version by prepending to your run command e.g.

NXF_VER=25.10.4 nextflow run ...

)

Hi. pipeline version is 2.17.0 and nextflow version is 26.04.0.

Could you test prepending

NXF_VER=25.10.4

because 26.04.0 came with sweeping changes and ampliseq wasnt tested with it.

Yes, I am trying it as we speak. Will let you know!

Alright, thanks. Will make 26.04.0 working with the next release 😉