GitHub
05/05/2026, 7:54 PMrandomreads.sh from BBMap with 100,000 reads each.
Three sample files were created from the random read files, sampling only 100 read pairs from each.
To make sure the three sample files map to slightly but not completely overlapping sets of genomes, they were created from the following genome read files:
`sourmash_10genomes_sample00_R{1,2}.fastq.gz`:
GCA_025449695.1
GCA_028291545.1
GCA_036004205.1
GCA_036270055.1
GCA_036380955.1
GCA_041659285.1
GCA_934196075.1
`sourmash_10genomes_sample01_R{1,2}.fastq.gz`:
GCA_025449695.1
GCA_028291545.1
GCA_036004205.1
GCA_036270055.1
GCA_944323475.1
GCF_010645065.1
GCF_964229035.1
`sourmash_10genomes_sample02_R{1,2}.fastq.gz`:
GCA_025449695.1
GCA_028291545.1
GCA_036380955.1
GCA_041659285.1
GCF_010645065.1
The three read files are referred to in samplesheets/sourmash_10genomes_samples.csv.
Sourmash index
A Sourmash index file (sourmash_10genomes.index.sbt.zip) was also made from the 10 genomes.
nf-core/test-datasetsGitHub
05/06/2026, 9:06 AMsourmash_24genomes.k31.index.sbt.zip and encodes the k-mer size in the filename, while the new one is sourmash_10genomes.index.sbt.zip. Worth keeping .k31. in there for consistency, but happy to leave it to your call.
Could you also close #2035 once this lands so the trail is clear?
nf-core/test-datasetsGitHub
05/06/2026, 9:23 AM