Hi everyone, as a new user I would just first like...
# oncoanalyser
m
Hi everyone, as a new user I would just first like to say thank you for the amazing tool. I wanted to run oncoanalyser from two different starting points. I have the normal samples already aligned, but not the tumor samples. However, I'm getting an error during validation where it says that it can't find the redux bams and tsvs in the same directory
Copy code
ERROR ~ When only specifying filetype BAM_REDUX in the sample sheet, make sure the REDUX BAM and TSVs are in the same dir:
<s3://bucket/oncoanalyser/PDO/alignments/dna/normal-sample.redux.bam> (BAM_REDUX)
/bucket/oncoanalyser/PDO/alignments/dna/normal-sample.jitter_params.tsv (missing expected REDUX_JITTER_TSV)
/bucket/oncoanalyser/PDO/alignments/dna/normal-sample.ms_table.tsv.gz (missing expected REDUX_MS_TSV)
Alternatively, provide the TSV paths in the sample sheet using filetype values: REDUX_JITTER_TSV, REDUX_MS_TSV
Is anything wrong with my samplesheet? Is it possible to have two different starting points for different sample types in the same samplesheet?
s
Hi @Mafalda Soares, this should be possible and your samplesheet looks correct at a glance
Could you provide any .nextflow.log, commands, and other information such as oncoanalyser and Nextflow version?
Can you also check that the real filepaths of each entry exist? I’m assuming that the filepaths in your samplesheet are for example only since they use
bucket
as the S3 Bucket name
m
Sorry, it was a mistake on my part, the real filepaths were not correct. Thank you for your help!
s
No worries, glad to hear it was an easy fix!