Hello everyone, I hope you're well. I prepare a metagenomic analysis for up to 100 sample. Assume that i didn't have access to HPC. Which material can you advise me ? seqera plateform or cloud...? Thanks.

Some analysis need not much more than a laptop with 100 samples (16S rRNA gene amplicon sequencing with a small taxonomic database), some need huge amounts of RAM & time (shotgun metagenomics co-assembly). So a little more details would help 😉

I want to run the nf-core/mag for mocrobiology sample

If you dont need https://nf-co.re/mag/5.4.2/parameters/#coassemble_group on your 100 samples and if you only assemble with MEGAHIT (short reads: skip spades) and possibly select your binners sensitively, you can reduce time & memory requirements substantially. However, how much remains is unclear, it depends strongly on your data. nf-core pipelines were tested on AWS and the sequera infrastructure, so they should work there. Resources such as https://www.denbi.de/ or https://clowm.bi.denbi.de/ help as well.

Thank🙌