Hej! I want to run ampliseq with older database versions of GTDB but have been running into some errors. My run fails with the database version sbdi-gtdb=R06-RS202-3 and sbdi-gtdb=R07-RS207-1. The errors are the same: "`Unable to access path:` " and then links to https://scilifelab.figshare.com/ndownloader/files/36980767 and https://scilifelab.figshare.com/ndownloader/files/31370437 . The files seem to exist on the links in the error messages. I suspect it is related to the thread started by Anders Johnson on Feb 13. That issue was resolved by using the latest ampliseq version, which has updated links to databases. I am already running version 2.16.1. Do you know how the issue can be solved?

Hi there, you could always pre-download the database files and use custom database input with parameters that include

ref_tax_custom

, e.g. https://nf-co.re/ampliseq/2.16.1/parameters/#dada_ref_tax_custom

Please make an issue for this, so I can fix the urls for older releases when I'm back from vacation