Anyone know how to submit “metabarcoding”/amplicon...
# meta-omics
j
Anyone know how to submit “metabarcoding”/amplicon sequencing data to ENA? I presume I should set an “environmental” taxon - e.g.
plant metagenome
? Do I need to submit demultiplexed data?
j
Calling @Martin Beracochea @Evangelos Karatzas who will know who to ask But to my knowledge yes to the taxon, and yes demultiplexed but only adapter removed (no further processing)
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Or maybe @Ekaterina Sakharova
e
@Jim Downie hi! I think you can use general ENA instructions how to submit reads. Example of metaB https://www.ebi.ac.uk/ena/browser/view/SRR27974960. Set correctly library layout, strategy and source ☝️ Pick taxon depending on your data. What is your data about? I think the recommendation is to demultiplex, yes
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j
Thanks, that's a really helpful example! I have 16S and ITS amplicon sequencing data from various plant tissues that I need to submit.