#1952 Add msproteomics test datasets
Pull request opened by
an-altosian
Summary
Public test datasets for the
nf-core/msproteomics pipeline.
•
TMT: PRIDE PXD000001 (Erwinia carotovora, TMT6) — 2 mzML subsets
•
DDA LFQ: Zenodo 1051552 (Human SILAC) — 2 mzML subsets
•
DIA: CPTAC CCRCC (Human DIA) — 1 mzML subset
•
FASTA: UniProt reference databases (Erwinia, Human SwissProt 2000-protein subset, E.coli+UPS1)
•
Module inputs: Pre-computed intermediate files for unit testing individual modules
•
Samplesheets: CSV inputs for all workflow test profiles
FASTA file sizes
| File | Size | Proteins |
| ------------------------ | ------ | -------- |
| ecoli_ups1_test.fasta | 1.8 MB | — |
| erwinia_carotovora.fasta | 1.6 MB | — |
| erwinia_uniprot.fasta | 1.9 MB | — |
| human_sp_subset.fasta | 1.4 MB | 2,000 |
human_sp_subset.fasta
is a smart subset: 169 proteins identified from the DDA LFQ test spectra (HEK SILAC) + 1,831 evenly-spaced SwissProt entries for search space diversity. Validated by running the full FragPipe DDA LFQ pipeline end-to-end (174 proteins identified at 1% FDR).
Supersedes
#1946 (closed due to force-push history issue).
🤖 Generated with
Claude Code
nf-core/test-datasets