Hi, can the output from nf-core: rnaseq (https://github.com/nf-core/rnaseq?tab=readme-ov-file) salmon.merged.gene_counts.tsv be used directly as sample input to this nf-core:differentialabundance pipeline? Thanks!

Hi, unfortunately I've been running into an error that I think is caused by my contrasts.csv. It may be coming from my not using a "blocking" variable , but I can't tell. Could it be because I don't have any biological replicates? Any tips? executor > slurm (6) [f0/8bb155] NFC…ssembly.annotation.gtf.gz) | 1 of 1 ✔️ [62/050a50] NFC…NCE:GTF_TO_TABLE (gencode) | 1 of 1 ✔️ [6a/3b79c8] NFC…ALIDATOR (samplesheet.csv) | 1 of 1 ✔️ [f6/5d2a7f] NFC…_MATRIXFILTER ([id:study]) | 1 of 1 ✔️ [d5/9687ad] NFC…et:condition2, blocking:]) | 0 of 1 ✘ [20/0bf543] NFC…et:condition2, blocking:]) | 0 of 1 [- ] NFC…ABUNDANCE:FILTER_DIFFTABLE - [- ] NFC…ABUNDANCE:PLOT_EXPLORATORY - [- ] NFC…BUNDANCE:PLOT_DIFFERENTIAL - [- ] NFC…TIALABUNDANCE:SHINYNGS_APP - [- ] NFC…BUNDANCE:RMARKDOWNNOTEBOOK - [- ] NFC…UNDANCE:MAKE_REPORT_BUNDLE - ERROR ~ Error executing process > 'NFCORE_DIFFERENTIALABUNDANCEDIFFERENTIALABUNDANCEDESEQ2_DIFFERENTIAL ([id:Patient1_vs_Patient2, variable:condition, re ference:condition1, target:condition2, blocking:])' Caused by: Process

NFCORE_DIFFERENTIALABUNDANCE:DIFFERENTIALABUNDANCE:DESEQ2_DIFFERENTIAL ([id:Patient1_vs_Patient2, variable:condition, reference:condition1, targ
et:condition2, blocking:])

terminated with an error exit status (1)

100%, none of these tools will function without biological replication I'm afraid.