Hello mag users ! What tool do you recommand for f...
# mag
s
Hello mag users ! What tool do you recommand for functional annotation of the MAGs retrieved with the pipeline. I was looking for something running Kofam, Pfam and Cazymes datasets like DRAM (having trouble running it right now)
j
Depends on exactly what you're looking for, but #C02K5GX2W93 will be relatively soon supporting CAZyme screening
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s
For now, I'm just looking to have a working pipeline from raw reads to functionally annotated MAGs
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j
@Evangelos Karatzas does #C084HP11VPF or something help with this somehow?
e
on the amino acid sequence level, yes. you'll have to pass the predicted proteins in a samplesheet and then run #C084HP11VPF. If you have multiple species from mag, you could first pass all the proteins through #C07URFZF074 first to scale down, and then use the produced family representative sequences only as input in #C084HP11VPF
j
I guess you could take the `faa`from prokka for it?