The docker image for

NFCORE_AMPLISEQ:AMPLISEQ:QIIME2_INSEQ

points to

<docker://qiime2/amplicon:2024.10>

and I am getting an error when singularity tries to pull this image:

ERROR ~ Error executing process > 'NFCORE_AMPLISEQ:AMPLISEQ:QIIME2_INSEQ'

Caused by:
  Failed to pull singularity image
    command: singularity pull  --name qiime2-amplicon-2024.10.img.pulling.1765477628027 <docker://qiime2/amplicon:2024.10> > /dev/null
    status : 255
    hint   : Try and increase singularity.pullTimeout in the config (current is "20m")
    message:
      FATAL:   While making image from oci registry: error fetching image: failed to get checksum for <docker://qiime2/amplicon:2024.10>: GET <https://index.docker.io/v2/qiime2/amplicon/manifests/2024.10>: UNAUTHORIZED: authentication required; [map[Action:pull Class: Name:qiime2/amplicon Type:repository]]

Going to https://hub.docker.com/u/qiime2, the amplicon image doesn't seem to be there? Is this a bug or something I am missing?

Hi, it seems to work for me and for github CI tests, ~12 hours back and now. The target is https://quay.io/repository/qiime2/amplicon?tab=tags and that contains 2024.10. The command to download the container with singularity should be

singularity pull  --name qiime2-amplicon-2024.10.img <docker://quay.io/qiime2/amplicon:2024.10> > /dev/null

, i.e. with quai.io which seems to be missing in the command that is used for you. Potentially that was overwritten by a config or such? You can try to pull the container manually:

singularity pull  --name qiime2-amplicon-2024.10.img <docker://quay.io/qiime2/amplicon:2024.10> > /dev/null

and if that works move it into

work/singularity/

so that it will be picked up when you start the pipeline. I just tested that locally and it worked for me.

For some reason my version does not pull from quay.io but directly from docker. When I run your script it works but if I run the singularity pull from the actual workflow, it fails

singularity pull  --name qiime2-amplicon-2024.10.img.pulling.1765477628027 <docker://qiime2/amplicon:2024.10> > /dev/null

Putting the .sif file from quay into my singularity cache dir worked though, so thank you!

Glad it works now!