Sara Terzoli
12/02/2025, 4:19 PMcellranger multi on some multiplexed single-cell data, using the --cellranger_multi_barcodes option. But I get this error:
We detected multiple probe barcodes in: Sample Lib_Flex_Multiplexing_INF-01 in "fastq_all/gex"
Singleplex Flex chemistry is invalid with >1 probe barcode. If this is a multiplex Flex library please include a [samples] section defining the inputs.
The following top probe barcodes were observed: BC001, BC002, BC003.
It looks like the [samples] section isn’t being created in my cellranger_multi_config.csv.
Any tips on how to fix this so it works with multiple probe barcodes?Felipe Almeida
12/21/2025, 9:55 PM