Hi All, I’m trying to run `cellranger multi` on s...
# scrnaseq
s
Hi All, I’m trying to run
cellranger multi
on some multiplexed single-cell data, using the
--cellranger_multi_barcodes
option. But I get this error:
Copy code
We detected multiple probe barcodes in: Sample Lib_Flex_Multiplexing_INF-01 in "fastq_all/gex"
Singleplex Flex chemistry is invalid with >1 probe barcode. If this is a multiplex Flex library please include a [samples] section defining the inputs.
The following top probe barcodes were observed: BC001, BC002, BC003.
It looks like the
[samples]
section isn’t being created in my
cellranger_multi_config.csv
. Any tips on how to fix this so it works with multiple probe barcodes?
f
Hi, Sorry for the delay here! Do you have examples of the CSVs you used and maybe the cellranger task log?