I have been running nf-core/mag 5.2.0 in offline mode using 4 ONT reds from http://www.inbirg.com/hlrmdb/ as a test. nextflow run nf-core/mag --skip_metabinner --input samplesheet.csv --outdir mag_out --gtdb_db /z/datasets/ds_mr_metagenome/nfcore_pipelines/data/gtdbtk_r226_data.tar.gz -profile sge,singularity -resume In NFCORE_MAG🔍*BIN_QC:CONCAT_BUSCO_TSV (busco) I encounter an error.* record on line 2: wrong number of fields The problem is that that the number of fields are not the same. In inputs/csv13/sample4-auto-busco.batch_summary.txt there is an additional field "internal stop codon percent” compared to inputs/csv12/sample1-auto-busco.batch_summary.txt. Can you guide me on how to proceed ? Best regards, Peter

This is a known bug, that should be fixed in a release coming this week ™️ (@Diego Alvarez Saravia)? You can try

-r dev

instead in teh meantime Maybe sure to

nextflow pull nf-core/mag first

Thanks for the quick reply James !

Unfortunately not unless you are ok with skippong BUSCO entirely and rely on just checkm/checkm2

I will be ok with skipping busco ! How can I do that ?

Uh actually I'm a bit confused, based on your command it shouldn't be running 🤔

Thanks !