Jiasheng
10/22/2025, 6:22 PMphenotype column in the samplesheet.csv for nf-core/raredisease v2.6.0.
For example, if the proband (ID = A001) is affected (phenotype = 2), and his father (ID = B001) is also affected, should I also set the father’s phenotype to 2? I’d like to confirm whether the pipeline assumes that there is only one affected individual per family/trio (i.e., only the proband should have phenotype = 2), or if multiple affected individuals within the same family are supported.
Many thanks in advance!