Florian Wuennemann
09/30/2025, 3:49 PMCommand error:
10:45PM INF instance metadata ami-id=ami-047eeab72a1800334 instance-id=i-01546edf255ede790 instance-type=r6id.4xlarge
[WARN tini (38)] Tini is not running as PID 1 and isn't registered as a child subreaper.
Zombie processes will not be re-parented to Tini, so zombie reaping won't work.
To fix the problem, use the -s option or set the environment variable TINI_SUBREAPER to register Tini as a child subreaper, or run Tini as PID 1.
/opt/conda/lib/python3.10/site-packages/anndata/_core/anndata.py:1756: UserWarning: Variable names are not unique. To make them unique, call `.var_names_make_unique`.
utils.warn_names_duplicates("var")
/usr/local/bin/read_data.py:63: UserWarning: No full resolution image found. If incorrect, please specify the path in the `fullres_image_file` parameter when calling the `visium_hd` reader function.
spatialdata = spatialdata_io.visium_hd(
Traceback (most recent call last):
File "/usr/local/bin/read_data.py", line 63, in <module>
spatialdata = spatialdata_io.visium_hd(
File "/opt/conda/lib/python3.10/site-packages/spatialdata_io/readers/visium_hd.py", line 308, in visium_hd
load_image(
File "/opt/conda/lib/python3.10/site-packages/spatialdata_io/readers/visium_hd.py", line 99, in load_image
_load_image(
File "/opt/conda/lib/python3.10/site-packages/spatialdata_io/readers/visium_hd.py", line 378, in _load_image
data = imread(path, **imread_kwargs)
File "/opt/conda/lib/python3.10/site-packages/dask_image/imread/__init__.py", line 48, in imread
with pims.open(sfname) as imgs:
File "/opt/conda/lib/python3.10/site-packages/pims/api.py", line 199, in open
raise UnknownFormatError(
pims.api.UnknownFormatError: All handlers returned exceptions:
<class 'pims.image_reader.ImageReader'> errored: Could not find a backend to open `Visium_HD_Human_Colon_Normal_P5/binned_outputs/square_002um/spatial/tissue_hires_image.png`` with iomode `r`.
Based on the extension, the following plugins might add capable backends:
pyav: pip install imageio[pyav]
opencv: pip install imageio[opencv]
<class 'pims.image_reader.ImageReaderND'> errored: Could not find a backend to open `Visium_HD_Human_Colon_Normal_P5/binned_outputs/square_002um/spatial/tissue_hires_image.png`` with iomode `r`.
Based on the extension, the following plugins might add capable backends:
pyav: pip install imageio[pyav]
opencv: pip install imageio[opencv]
<class 'pims.imageio_reader.ImageIOReader'> errored: Could not find a backend to open `Visium_HD_Human_Colon_Normal_P5/binned_outputs/square_002um/spatial/tissue_hires_image.png`` with iomode `r?`.
Based on the extension, the following plugins might add capable backends:
pyav: pip install imageio[pyav]
opencv: pip install imageio[opencv]Christophe Avenel
10/01/2025, 12:18 PMtissue_hires_image.png.
It claims that adding the imageio[pyav] or imageio[opencv] pip package could fix it. Could you share the png file in Visium_HD_Human_Colon_Normal_P5/binned_outputs/square_002um/spatial/tissue_hires_image.png so that I can check the problem?