Hello, Thank you for creating the pipeline. As a n...
# denovotranscript
s
Hello, Thank you for creating the pipeline. As a newcomer to nf-core, I am attempting to run this pipeline for de novo assembly of Oak transcripts. I was able to successfully execute the pipeline up to the Trinity and rnaSPAdes steps, but it consistently fails at the
tr2aacds.pl
step. Specifically, the issue seems to be with the BLAST commands. I have attached the log file and a screenshot of the directory where the temporary BLAST outputs are being stored. Could you please let me know what might be causing this error?