Hello, I received my CLIPseq data. First step is to demultiplex. The software runs just fine (ultraplex) but I have questions. The plateform has trimmed the reads so now it should begin with the UMI (NNNN) then the 8 barcodes caracteres then again NN that just separate the reads and the barcodes (from 5' to 3'). When I give the barcode for demultiplexing with this form NNNNXXXXXXXXNN, the software put 6 N as UMI (the 4 first that are the UMI and the 2N at the end that plays separators). Is that correct ? I would say that it's not a probleme for deduplication but as I've never done that before I'm a bit naive and maybe I don't anticipate a probleme in doing it that way. What do you think please ? Also, I was wondering the number of mismatch to allow for the barcodes. If I set it at 2 is that enougth (barcodes of 8 caracteres and 6 conditions to separate) ? Is it too much ? Do I have to take into account the differences between the barcodes to choose ? What are usually the criteria to decide please ? At last, is there any other parameters that should retain my attention or can I leave it to default ? Like the reads qualité maybe ? I appreciate any help, I'm eager to learn here but Google is my only help and it only goes so far... Best,