I would also agree that introducing a length filter too early would be detrimental for understanding or interpreting certain experiments. Here are some examples of public datasets that would be affected (even though arguably not too badly):
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C Z
06/17/2025, 1:11 PM
% of total reads are ribosome protected fragments based on periodicity > Ribotricer phase score?
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Felix Krueger
06/17/2025, 1:14 PM
These stats are taken from the Ribotish results for each read length
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C Z
06/17/2025, 1:21 PM
Sorry, bit confused here... >30% of reads that are 29nt were considered RPFs? These values were found in riboseq_qc -> ribotish folder?
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Felix Krueger
06/17/2025, 1:28 PM
yes, that folder.
Felix Krueger
06/17/2025, 1:30 PM
and the number be: all mapped fragments between 25-35nt in length make up the total, the fraction being 29 or 30nt is 30%. (or in other words, all read lengths <26 and >31 amount to less than 10% or so in total