Hi, quick question. Have someone used airrflow pip...
# airrflow
r
Hi, quick question. Have someone used airrflow pipeline for Smart-seq data or other well-based single-cell sequencing? We are doing a test from from a non-targetted gene expression library to see if we can get heavy and light chain paired up.
I not doing it for human and mouse data either, so I need to be able to input a custom database.
m
w.r.t. single cell: airrflow can run the process from fastq files if the data comes from 10x genomics. Otherwise you can give AIRR-seq files as input for airrflow
r
I see, so it won't separate each cell based on their barcode if it is not 10x.
m
yes, for sc sequence alignment it currently only supports 10x
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r
Thank for the fast responses, though!
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