Hi, quick question. Have someone used airrflow pipeline for Smart-seq data or other well-based single-cell sequencing? We are doing a test from from a non-targetted gene expression library to see if we can get heavy and light chain paired up.
Rodrigo Arcoverde
01/22/2025, 2:31 PM
I not doing it for human and mouse data either, so I need to be able to input a custom database.
m
Mark Polster
01/22/2025, 2:34 PM
w.r.t. single cell:
airrflow can run the process from fastq files if the data comes from 10x genomics.
Otherwise you can give AIRR-seq files as input for airrflow
r
Rodrigo Arcoverde
01/23/2025, 10:57 AM
I see, so it won't separate each cell based on their barcode if it is not 10x.
m
Mark Polster
01/23/2025, 12:45 PM
yes, for sc sequence alignment it currently only supports 10x