I’m also not getting this pipeline to work. Here i...
# circrna
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I’m also not getting this pipeline to work. Here is my test `samplesheet.csv`:
Copy code
cat samplesheet.csv
sample,fastq_1,fastq_2
cel_N2_1,<https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/N2_rep1_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/N2_rep1_2.fastq.gz>
cel_N2_2,<https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/N2_rep2_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/N2_rep2_2.fastq.gz>
cel_N2_3,<https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/N2_rep3_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/N2_rep3_2.fastq.gz>
fust1_1,<https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/fust1_rep1_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/fust1_rep1_2.fastq.gz>
fust1_2,<https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/fust1_rep2_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/fust1_rep2_2.fastq.gz>
fust1_3,<https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/fust1_rep3_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/circrna/fastq/fust1_rep3_2.fastq.gz>
and my `params.yaml`:
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cat params.yaml
# <https://nf-co.re/circrna/dev/parameters/>
input: 'samplesheet.csv'
outdir: 'results'
#gtf: 'references/gencode_v27_annotation.gtf'
#fasta: 'references/GRCh38_primary_assembly_genome.fa'
#genome: 'GRCh38'
#profile: 'docker'
email: 'leendertcloete@gmail.com'
multiqc_title: 'test_circRNA_nfcore'
tools: 'ciriquant,circexplorer2,find_circ,circrna_finder,mapsplice,dcc'
bsj_reads: 5
min_tools: 3
And with this cmd:
docker run -it --rm -v /var/run/docker.sock:/var/run/docker.sock -v $(pwd):$(pwd) -w $(pwd) nextflow/nextflow:24.10.3 bash -c 'nextflow run -r dev nf-core/circrna -params-file params.yaml -profile docker --genome GRCh38'
I get this error. What am I doing wrong here?