Hi everyone! The pipeline failed with the followin...
# riboseq
r
Hi everyone! The pipeline failed with the following message:
Copy code
executor >  slurm (116)
[34/1b96de] NFC…OME:GTF_FILTER (genome.fa) | 1 of 1 ✔
[c6/23457d] NFC…PTS_FASTA (rsem/genome.fa) | 1 of 1 ✔
[2e/f24243] NFC…_GETCHROMSIZES (genome.fa) | 1 of 1 ✔
[af/8bd231] NFC…ENOME:SORTMERNA_INDEX ([]) | 1 of 1 ✔
[-        ] NFC…_SETSTRANDEDNESS:CAT_FASTQ -
[b4/634dc4] NFC…RANDEDNESS:FQ_LINT (WTRPF) | 22 of 22 ✔
[57/4ef323] NFC…IMGALORE:FASTQC (WTBTOTAL) | 22 of 22 ✔
[54/0bd6c4] NFC…GALORE:TRIMGALORE (WTBRPF) | 22 of 22 ✔
[f2/36dc06] NFC…NT_AFTER_TRIMMING (WTBRPF) | 22 of 22 ✔
[49/c66e39] NFC…DNESS:SORTMERNA_INDEX ([]) | 1 of 1 ✔
[f9/caf96d] NFC…DNESS:SORTMERNA (33661RPF) | 20 of 24, failed: 5, retries: 2
[-        ] NFC…SS:FQ_LINT_AFTER_SORTMERNA -
[-        ] NFC…PLE_FQ_SALMON:SALMON_INDEX -
[-        ] NFC…PLE_FQ_SALMON:FQ_SUBSAMPLE -
[-        ] NFC…PLE_FQ_SALMON:SALMON_QUANT -
[-        ] NFC…ASTQ_ALIGN_STAR:STAR_ALIGN -
[-        ] NFC…TOOLS_GENOME:SAMTOOLS_SORT -
[-        ] NFC…OOLS_GENOME:SAMTOOLS_INDEX -
[-        ] NFC…TS_SAMTOOLS:SAMTOOLS_STATS -
[-        ] NFC…SAMTOOLS:SAMTOOLS_FLAGSTAT -
[bd/fe31b4] NFC…OTRICER_PREPAREORFS (null) | 1 of 1 ✔
Plus 18 more processes waiting for tasks…
ERROR ~ Error executing process > 'NFCORE_RIBOSEQ:RIBOSEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:SORTMERNA (13_85TOTAL_RNA)'

Caused by:
  Process `NFCORE_RIBOSEQ:RIBOSEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:SORTMERNA (13_85TOTAL_RNA)` terminated for an unknown reason -- Likely it has been terminated by the external system


Command executed:

  sortmerna \
      --ref rfam-5.8s-database-id98.fasta --ref rfam-5s-database-id98.fasta --ref silva-arc-16s-id95.fasta --ref silva-arc-23s-id98.fasta --ref silva-bac-16s-id90.fasta --ref silva-bac-23s-id98.fasta --ref silva-euk-18s-id95.fasta --ref silva-euk-28s-id98.fasta \
       \
      --reads 13_85TOTAL_RNA_1_val_1.fq.gz --reads 13_85TOTAL_RNA_2_val_2.fq.gz \
      --threads 12 \
      --workdir . \
      --aligned rRNA_reads --fastx --other non_rRNA_reads \
      --paired_in \
      --out2 \
      --num_alignments 1 -v --index 0
  
  
          mv non_rRNA_reads_fwd.f*q.gz 13_85TOTAL_RNA_1.non_rRNA.fastq.gz
          mv non_rRNA_reads_rev.f*q.gz 13_85TOTAL_RNA_2.non_rRNA.fastq.gz
          mv rRNA_reads.log 13_85TOTAL_RNA.sortmerna.log
  
  
  cat <<-END_VERSIONS > versions.yml
  "NFCORE_RIBOSEQ:RIBOSEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:SORTMERNA":
      sortmerna: $(echo $(sortmerna --version 2>&1) | sed 's/^.*SortMeRNA version //; s/ Build Date.*$//')
  END_VERSIONS

Command exit status:
  -

Command output:
  Found flag: --num_alignments
  Found value: 1 of previous flag: --num_alignments
  Found flag: -v
  Previous flag: -v is Boolean. Setting to True
  Found flag: --index
  Found value: 0 of previous flag: --index
  [opt_workdir:995] Using WORKDIR: "." as specified
  [process:1483] Processing option: aligned with value: rRNA_reads
  [process:1483] Processing option: fastx with value: 
  [process:1483] Processing option: index with value: 0
  [opt_index:1182] using 'index' with specified value 0
  [process:1483] Processing option: num_alignments with value: 1
  [process:1483] Processing option: other with value: non_rRNA_reads
  [process:1483] Processing option: out2 with value: 
  [process:1483] Processing option: paired_in with value: 
  [process:1483] Processing option: reads with value: 13_85TOTAL_RNA_1_val_1.fq.gz
  [opt_reads:98] Processing reads file [1] out of total [2] files
  [process:1483] Processing option: reads with value: 13_85TOTAL_RNA_2_val_2.fq.gz
  [opt_reads:98] Processing reads file [2] out of total [2] files
  [process:1483] Processing option: ref with value: rfam-5.8s-database-id98.fasta
  [opt_ref:158] Processing reference [1] out of total [8] references
  [opt_ref:206] File "rfam-5.8s-database-id98.fasta" exists and is readable
  [process:1483] Processing option: ref with value: rfam-5s-database-id98.fasta
  [opt_ref:158] Processing reference [2] out of total [8] references
  [opt_ref:206] File "rfam-5s-database-id98.fasta" exists and is readable
  [process:1483] Processing option: ref with value: silva-arc-16s-id95.fasta
  [opt_ref:158] Processing reference [3] out of total [8] references
  [opt_ref:206] File "silva-arc-16s-id95.fasta" exists and is readable
  [process:1483] Processing option: ref with value: silva-arc-23s-id98.fasta
  [opt_ref:158] Processing reference [4] out of total [8] references
  [opt_ref:206] File "silva-arc-23s-id98.fasta" exists and is readable
  [process:1483] Processing option: ref with value: silva-bac-16s-id90.fasta
  [opt_ref:158] Processing reference [5] out of total [8] references
  [opt_ref:206] File "silva-bac-16s-id90.fasta" exists and is readable
  [process:1483] Processing option: ref with value: silva-bac-23s-id98.fasta
  [opt_ref:158] Processing reference [6] out of total [8] references
  [opt_ref:206] File "silva-bac-23s-id98.fasta" exists and is readable
  [process:1483] Processing option: ref with value: silva-euk-18s-id95.fasta
  [opt_ref:158] Processing reference [7] out of total [8] references
  [opt_ref:206] File "silva-euk-18s-id95.fasta" exists and is readable
  [process:1483] Processing option: ref with value: silva-euk-28s-id98.fasta
  [opt_ref:158] Processing reference [8] out of total [8] references
  [opt_ref:206] File "silva-euk-28s-id98.fasta" exists and is readable
  [process:1483] Processing option: threads with value: 12
  [process:1483] Processing option: v with value: 
  [process:1503] === Options processing done ===
  [process:1504] Alignment type: [best:1 num_alignments:1 min_lis:2 seeds:2]
  [validate_kvdbdir:1248] Key-value DB location "./kvdb"
  [validate_kvdbdir:1284] Creating KVDB directory: "./kvdb"
  [validate_idxdir:1214] Using index directory: "/gpfs/commons/home/rmassoni/cll_btk_resistance/current/CLLU1/work/9e/79488f8f8f0ebb4d2d969a8487abe6

Command error:
  INFO:    Mounting image with FUSE.

Work dir:
  /gpfs/commons/home/rmassoni/cll_btk_resistance/current/CLLU1/work/9e/79488f8f8f0ebb4d2d969a8487abe6

Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out`

 -- Check '.nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: <https://nf-co.re/docs/usage/troubleshooting>

 -- Check '.nextflow.log' file for details
I tried searching the error but nothing clear came out. Could you please help me troubleshoot it? Thanks!
j
This message:
Copy code
Process `NFCORE_RIBOSEQ:RIBOSEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:SORTMERNA (13_85TOTAL_RNA)` terminated for an unknown reason -- Likely it has been terminated by the external system
... suggests an issue native to your local infrastructure. You'd need to work out why that node killed the job
👍 1
r
thanks Jonathan