Hi! I just found this pipeline, looks nice! Can I ...
# denovotranscript
t
Hi! I just found this pipeline, looks nice! Can I expect this to run successfully already even though it's still in development?
a
Yes, it should work. Keen to see people using it!
I haven't set up a DOI for the pipeline yet for citation, so you might need to check back for that
t
Ok!
It's already running.
🙌 1
Hmm, Trinity died with segfault, probably has nothing to do with the pipline though.
t
Could be a memory issue, if you are able try running once or twice more to see if it happens again.
with more mem available if you are able
t
Not sure, it seems to happen already at the normalization stage.
I'll look into it tomorrow...
j
I also found the pipeline and testing it right now! I have a huge RNAseq dataset so I skip Trinity because I know it will run for weeks... But I also got memory issues with Spades, so I created a custom config file like this:
Copy code
process {
    withName: SPADES {
      cpus = 32
      memory = 170.GB
  }
}
It's running now, but not sure it will complete...