Zachary Henning
07/05/2024, 4:51 PMCaused by:
Process `NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH (megahit.prodigal)` terminated with an error exit status (1)
Command executed:
rc=0
mkdir contigs
gunzip -c megahit.prodigal.faa.gz > ./contigs/proteins.faa
EUKulele \
-m mets \
--database gtdb \
--protein_extension .faa \
--reference_dir eukulele \
-o megahit.prodigal \
--CPUs 48 \
-s \
contigs || rc=$?
gzip megahit.prodigal/mets_full/diamond/*.out
gzip megahit.prodigal/taxonomy_counts/*.csv
gzip megahit.prodigal/taxonomy_estimation/*.out
cat <<-END_VERSIONS > versions.yml
"NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH":
eukulele: $(echo $(EUKulele --version 2>&1) | sed -n 's/.* \([0-9]\+\.[0-9]\+\.[0-9]\+\).*/\1/p')
END_VERSIONS
if [ $rc -le 1 ]; then
exit 0
else
exit $rc;
fi
Command exit status:
1
Command output:
All reference files for GTDB downloaded to eukulele/gtdb
Running EUKulele with command line arguments, as no valid configuration file was provided.
Setting things up...
Could not successfully install all external dependent software.
Check DIAMOND, BLAST, BUSCO, and TransDecoder installation.
['proteins']
Specified reference directory, reference FASTA, and protein map/taxonomy table not found. Using database in location: eukulele/gtdb.
Automatically downloading database gtdb . If you intended to use an existing database folder, be sure a reference FASTA, protein map, and taxonomy table are provided. Check the documentation for details.
Command error:
Matplotlib created a temporary config/cache directory at /tmp/SLURM_24325473/matplotlib-w5yqnp9o because the default path (/home1/zjhennin/.config/matplotlib) is not a writable directory; it is highly recommended to set the MPLCONFIGDIR environment variable to a writable directory, in particular to speed up the import of Matplotlib and to better support multiprocessing.
mkdir: can't create directory 'eukulele/': No such file or directory
mkdir: can't create directory 'eukulele/': No such file or directory
eukulele/gtdb/reference.pep.fa: No such file or directory
eukulele/gtdb/taxonomy-table.txt: No such file or directory
gzip: megahit.prodigal/mets_full/diamond/*.out: No such file or directory